Malign Melanomlar ile Melanositik Nevüs Ayrımında EGFR, CCND1 ve RREB1 Genlerini Hedef Alan FISH Testinin Yeri

Melanocytic nevus (MN) may on occasion be difficult to distinguish from malignant melanoma (MM) histopathologically. Fluorescent in situ hybridization (FISH) has been demonstrated of use for the diagnosis melanocytic neoplasms skin. In this study, effectiveness standart FISH test (4-way probe targeting RREB1, CCND1, MYB genes and centromere 6) additionally probes, targetting EGFR, TP53, MDM2 TP53 genes, differentiating melanomas nevi were investigated. Standard was performed 24 MM MN samples, but gene copy numbers investigated 16 using method. The incidence FISH-detected positive genomic aberrations probe, others) determined as 83,3% cases, 5,2% MN. Statistically significant differences found between groups terms EGFR amplifications (p<0.001, p<0.05, p˂0.05), there no association histopathological features detected abnormalities (p>0,05). additionally, all 5 acral lentiginous melanomas, could analysed, had amplifications. conclusion, have diagnostic significance MM. is very effective its an adjunct methods. But controlled probes should used avoid false results.